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Anjali Shiras

Adjunct Professor, S.P. University of Pune, India

Characterization of independent clones of human induced pluripotent stem cell-line-NCCSi005 derived from control individual of indian ethnicity

Human induced pluripotent stem cell-line (iPSC) of Indian origin NCCSi005 was established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of healthy female donor. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Three independent clones derived from NCCSi005- NCCSi005A, NCCSi005B and NCCSi005C were independently chosen for study and RNA-seq analyses. The presentation elaborates a detailed characterization of each of the three clones with reference to their stemness markers and potential for tri-lineage differentiation, along with their functional differentiation to the cardiomyocyte lineage. The RNA sequencing analyses of each of the three independent clones indicated similar gene expression profile indicating homogeneity amongst these clones. Our data indicated that NCCSi005 cell-line represents a valuable resource as control human iPSC cell line of Indian origin.


Biography

Dr. Anjali Shiras received her Ph.D from University of Mumbai, India in field of cancer biology. She soon secured a faculty position at National Centre for Cell Science in Pune where she opened her independent lab. She took her sabbatical break and worked with Dr. Meenhard Herlyn in area of melanoma biology at Wistar Institute, Philadelphia, USA. Science in her lab is driven towards understanding mechanisms of cell proliferation and growth, key questions that underlies our existence. She works on understanding mechanisms through which long noncoding RNAs mediate pervasive transcription and yet stay at the helm of affairs for maintaining gene regulation in mouse and human cells. Her group is exploring signalling at the level of miRNAs, long noncoding RNAs, exosomes and signalling mechanisms pertaining genomic instability to understand tumor progression in glioblastoma. A major focus of her lab is on studying the biology of human induced pluripotent stem cells. She is involved in establishing a core banking facility for Induced Pluripotent Stem Cells of human origin. By using Nex-Gen sequencing approaches her group is involved in generating gene signatures that would aid in disease modelling and drug screening applications. In this presentation, she discusses the approaches used by her group for generating and characterizing a control human iPSC line of Indian origin.

Faculty / Academic Appointments

1990- Present:         National Centre for Cell Science, Pune, India. 

 Principal Investigator and Scientist G- National Centre for Cell Science, 

 Pune,  India

2013- Present:       Adjunct Professor at S.P. University of Pune in

                                  Bio-chemisty, Chemistry and Bioinformatics S.P. University of Pune 

Education & Training

1985 – 1990    :  Ph. D.   (Applied Biology) -  Cancer Research Institute, Mumbai University

1982 - 1984     :   M.Sc    (Microbiology)     –   Ranked 2nd in Pune University

1979 – 1982    :   B.Sc     (Microbiology)     -     Ranked 1st in Mumbai University

Fellowships and Memberships of Professional Bodies

2011-12      :   DBT Crest Fellowship availed with Dr. Meenhard  Herlyn;  Director, Melanoma 

                         Research program; Wistar Institute, Philadelphia, USA.

2008         :   Member of the Panelist in the Asia-Pacific Networking Meeting held at Tokyo, Japan 

2015           :   Part of Prime minister’s delegation to Japan for Formulating joint research activities with Japan 

2013, 2012, 2010,2006 and 2005: Travel awards from International Society for Stem Cell research (ISSCR); USA.

2014 ,2016 : Delegate for Training and Mentoring African Scientists in Stem Cell and Regenerative Medicine Research from 4 to 6 August 2014 at the AAS secretariat in Nairobi, Kenya and Cape Town, South Africa.

Memberships of Professional Bodies

•    International Society for Stem Cell Research (ISSCR), USA

•    Indian Society of Neuro-oncology (ISNO), India (Executive Council, Member)

•    Indian Association of Cancer Research (IACR), India  (Executive Council, Member)


       List of Important Publications

1.     Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+T cells of healthy individuals of Indian origin. Khan M, Zende S, Vaidyanath A, Avatade R, *Shiras A. Stem Cell Res. 2019 Jul 18;39:101506

2.     Attenuation of Tumor Suppressive Function of FBXO16 Ubiquitin Ligase Activates Wnt signaling    in Glioblastoma. Khan M, Muzumdar D, *Shiras A. Neoplasia. 2019 Jan;21(1):106-116

3.   Noncoding RNA Ginir Functions as an Oncogene by Associating with Centrosomal Proteins; Suchismita Panda, Meenakshi Setia, Navjot Kaur, Varsha Shepal ,Vivek Arora, Divya Kumari Singh, Abir Mondal, Abhishek Teli,  Madhura Tathode, Rajendra Gajula, L.C. Padhy, *Anjali Shiras. Plos Biol, 2018; Oct 8;16(10):e2004204.

4.    Angiogenic Gene Signature Derived from Subtype Specific Cell Models Segregate Proneural and Mesenchymal Glioblastoma. Sharma A, Bendre A, Mondal A, Muzumdar D, Goel N, *Shiras A. Front Oncol. 2017 Jul 11;7:146. 

5.    Tumor suppressive miRNA-34a suppresses cell proliferation and tumor growth of glioma stem cells by targeting Akt and Wnt signaling pathways. Rathod SS, Rani SB, Khan M, Muzumdar D, *Shiras A. FEBS Open Bio. 2014 May 22;4:485-95.

6.    MiR-145 functions as a tumor-suppressive RNA by targeting Sox9  and adducin 3 in human glioma cells; Sandhya B. Rani†, Sachin Shivaji Rathod, Shanmuganandam Karthik, Navjot Kaur, Dattatraya Muzumdar, and *Anjali S. Shiras. Neuro Oncol. 2013 Oct;15(10):1302-16. 

7.    Wnt3a mediated activation of Wnt/β-catenin signaling promotes tumor progression in glioblastoma. Kaur N, Chettiar S, Rathod S, Rath P, Muzumdar D, Shaikh ML, *Shiras A.  Mol Cell Neurosci. 2013 Jan 19;54C:44-57.

8.    Epigenetic regulation of DNA methyltransferases: DNMT1 and DNMT3B in gliomas. Rajendran G,  Shanmuganandam K, Bendre A, Mujumdar D, Goel A, *Shiras A. J Neurooncol.  2011 Sep;104 (2):483-94. 

9.    Dlxin-1, a MAGE family protein, induces accelerated neurite outgrowth and cell survival by enhanced and early activation of MEK and Akt signalling pathways in PC12 cells. Reddy EM, Chettiar ST, Kaur N, Shepal V, *Shiras A. Exp Cell Res. 2010 Aug 15;316(14):2220-36. 

10.    Spontaneous transformation of human adult nontumorigenic stem cells to cancer stem cells is driven by genomic instability in a human model of glioblastoma. *Shiras A, Chettiar ST, Shepal V, Rajendran G, Prasad GR, Shastry P. Stem Cells. 2007 Jun;25(6):1478-89. 


•    Total Number of Publications (published or in Press) :    40

•    Total number of Citations                                            :    1500

•    H-index                                                                       :     19

 

Academic Interests

•    Cancer Biology: Biology of Glioma, Cellular Signalling, Glioma Stem Cells, microRNAs and long noncoding RNAs in Glioma pathogenesis.

•    Stem Cell Biology: Induced Pluripotent Stem Cells and Mouse Embryonic Stem cells

•    RNA Biology: Functional Annotation of Mouse and Human Linc RNAs 

Area of Specialization

•    Cancer Biology

•    Cell-line Generation from Normal and Tumor Tissues. 

•    Generation of Induced Pluripotent Stem Cell-lines

•    Exosome Biology 

•    Cancer Stem Cells 

•    Long noncoding RNAs and micro RNAs in Cancer.