Step 1 - Tool design
Thermo Fisher Scientific
Designing the genome editing strategies for stem cells
The customer can expect to learn the basic steps involved in designing a genome editing experiment. They will be exposed to the criteria involved in deciding whether to choose CRISPR or TALEN for an editing workflow. They will also learn how to design the donor template for the editing workflow.
Previous to his current tenure as a Scientist in the Cell Biology business of Thermo Fisher Scientific, Sanjay Kumar accumulated > 10 years of experience studying the cellular response to DNA damage. That work began with Sanjay’s pursuit of his doctorate degree, resulting in his PhD from the Cancer Genetics Department of the Roswell Park Cancer Institute in Buffalo NY. At Roswell, Sanjay identified the mechanism of replication fork slowdown upon DNA damage in fission yeast. His work in the DNA damage field continued with postdoctoral research at Caltech in Pasadena CA where he dissected the role of the Rif1 protein in the vertebrate intra-S-phase checkpoint. Additionally, Sanjay holds a business degree in Bioscience Management from the Keck Graduate Institute. As part of the scientific team at Thermo Fisher Scientific, he has contributed to the commercial development of Genome Editing tools such as TALENs and CRISPRs. He played a key role in the development of the GeneArt® CRISPR Nuclease mRNA. Currently he is leading the Custom Cell Line Engineering team within the Custom Services, this role requires Sanjay to guide his team in executing several custom cell engineering projects. Sanjay is also involved in streamlining Cell Engineering workflows for a variety of applications.
Transfection using the Neon Transfection System
The Neon transfection system enables robust transfection efficiencies in hiPSC for DNA, mRNA and Cas9/gRNA RNP complexes. For hiPSC these improved transfection as well as post-electroporation culture conditions are essential for increased cell survival for downstream applications. This live training will review protocols involving Neon electroporation of hiPSC and post electroporation culture, using delivery of CRISPR/Cas9-mediated genome editing tools as the example.
Jackie completed her undergraduate degree BSc (Biochemistry) at UCLA. After her degree, she spent a year at a biotech/computer science startup in South San Francisco, California and then joined Thermo Fisher Scientific as a Scientist on the Custom Driven Biology Services Team in Carlsbad, California, where she is responsible for many projects involving the hiPSC and neuronal product portfolios.View Month