Step 2 - Cloning
Thermo Fisher Scientific
This tutorial will cover experimental details for the use of FACS to clonally isolated edited pools of iPSCs. Cell dissociation, staining, cell preparation for sorting, sort gating and single cell plating will be covered.
Garrett Wong joined Thermo Fisher Scientific in February of 2018 after completing a Master’s in Biological Sciences from UCSD focusing on disease modeling using cells differentiated from iPSCs. He continues to work on different stem cell related projects for the Carlsbad, Californiabased custom biology services team at Thermo Fisher Scientific.