Edwin Golez

Thermo Fisher Scientific

3D neural organoid formation from iPSCs

Advances in cell culture techniques have focused on creating 3-dimensional (3D) systems in an attempt to represent in vivo cell–cell relationships and microenvironments in vitro utilizing adult and pluripotent stem cells (ASCs and PSCs). Starting with a PSC culture, we aggregate the cells to form an embryoid body (EB), when given the proper cues, will undergo self-differentiation and morphogenesis that better recapitulates the in vivo cell to cell interactions and microanatomy of a given tissue type. The development of a 3D organ-like (organoid) system requires the application of growth factors, small molecules, and other media supplements to guide the formation of organoid systems based on the embryogenesis and adult stem cell biology principles. In this training video, we will show you how to develop 3D neural organoids from iPSC culture utilizing a feeder-free system, our Gibco Geltrex matrix, and Thermo Nunclon Sphera microplates.

Cancer spheroid formation using Nunclon Sphera microplates

2D cultured cells can differ in terms of both physiology and cellular responses compared with cells in vivo. Mounting evidence suggests that culturing cells in 3D is more representative of the in vivo environment, creating more physiological cell models. Spheroids, or sphere cultures, have become especially popular area of 3D in vitro culture due to there great potential for use in studies that investigate growth and function of both normal and malignant tissues. Spheroids offer particular benefits in cancer biology, where they contribute immense value in examining the growth and behavior of tumors since they share several key histomorphological and functional traits that include the formation of cell-cell contacts, decreased proliferation, increased survival rates, and a hypoxic core. In this training video, we will demonstrate how to create 3D cancer spheroids using Nunclon Sphera 96 well U-bottom microplates.

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