National Centre for Cell Science
Characterization of Independent Clones of Human Induced Pluripotent Stem Cell-line-NCCSi005 derived from Control Individual of Indian Ethnicity
Human induced pluripotent stem cell-line (iPSC) of Indian origin NCCSi005 was established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of healthy female donor. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Three independent clones derived from NCCSi005- NCCSi005A, NCCSi005B and NCCSi005C were independently chosen for study and RNA-seq analyses. The presentation elaborates a detailed characterization of each of the three clones with reference to their stemness markers and potential for tri-lineage differentiation, along with their functional differentiation to the cardiomyocyte lineage. The RNA sequencing analyses of each of the three independent clones indicated similar gene expression profile indicating homogeneity amongst these clones. Our data indicated that NCCSi005 cell-line represents a valuable resource as control human iPSC cell line of Indian origin.
Dr. Anjali Shiras received her Ph.D from University of Mumbai, India in field of cancer biology. She soon secured a faculty position at National Centre for Cell Science in Pune where she opened her independent lab. She took her sabbatical break and worked with Dr. Meenhard Herlyn in area of melanoma biology at Wistar Institute, Philadelphia, USA. Science in her lab is driven towards understanding mechanisms of cell proliferation and growth, key questions that underlies our existence. She works on understanding mechanisms through which long noncoding RNAs mediate pervasive transcription and yet stay at the helm of affairs for maintaining gene regulation in mouse and human cells. Her group is exploring signalling at the level of miRNAs, long noncoding RNAs, exosomes and signalling mechanisms pertaining genomic instability to understand tumor progression in glioblastoma. A major focus of her lab is on studying the biology of human induced pluripotent stem cells. She is involved in establishing a core banking facility for Induced Pluripotent Stem Cells of human origin. By using Nex-Gen sequencing approaches her group is involved in generating gene signatures that would aid in disease modelling and drug screening applications. In this presentation, she discusses the approaches used by her group for generating and characterizing a control human iPSC line of Indian origin.